Numbers are updated and entered into the rotating numerical rings of each of the 5 slots using the 5 keys which look pretty like much like the keys of a typewriter. Each slot contains three rotating numeral rings from 0-9. A typical cell counter contains 5 counting slots representing the 5 types of wbc’s, along with 1 totalling slot that automatically adds up the numbers in the 5 slots into a total count. Now switch to the 100x oil immersion objective of the microscope.īefore you actually begin the counting, prepare on a blank sheet of paper, any one of the two types of tables shown here.īetter still, You can make use of a handy piece of tool called the blood cell counter. Once you have the stained blood smear dried and ready, place the slide on the microscope stage and first observe under low power of the microscope to get an idea of the the distribution of the blood cell components on the slide. To begin the DLC procedure, first prepare a thin peripheral blood smear of the given blood sample and stain it with an appropriate dye solution. Additionally, a blood cell counter may be used if available. The DLC procedure is a pretty simple process and can be performed in a modest lab setting.Īll that is required to perform a DLC are a compound light microscope and a thin stained peripheral blood smear of the patients blood sample, along with a pen and paper. The DLC is usually expressed in terms of percentage of that particular type of wbc. The differential leucocyte counting is an important biomedical tool used to quantify the number of each type of WBC or leucocyte.
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